Pertanyaan
What enzyme does the ONPG test use to allow organisms to be classified as lactose fermenters?
Jawaban
Penjelasan
Step1:
Understanding the ONPG Test The ONPG test is a biochemical test used to determine if an organism can ferment lactose. It involves the use of ONPG (ortho-Nitrophenyl-β-galactoside), which is a substrate for the enzyme β-galactosidase.
Step2:
Role of β-galactosidaseβ-galactosidase is the enzyme that breaks down lactose into glucose and galactose. If an organism produces β-galactosidase, it will hydrolyze ONPG, resulting in a color change due to the release of ortho-nitrophenol, indicating lactose fermentation.
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